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Accurately take a certain amount of sample in the PTFE liner of a suitable digester and shake it several times after adding a certain amount of digestion reagent to make the sample and the reagent evenly contacted. Put inside digestion tank and cover. The digester was placed on a tray in a microwave digestion oven. According to different samples set microwave heating power, heating time and control pressure for digestion. After the digestion is complete, cool it down and take it out.
Through the use of this digester, it has been found that biological samples often contain fat. When digested by dry or wet methods, it takes a long time (4 to 6 hours) to digest, but it is not easily digested completely. Microwave digestion can shorten the time, but the digestion reaction of fat and digestion reagents is very severe, and the evolved gas often leads to overflow or even explosion. The author used the sample plus the digestion reagent and then pre-digested it in the water bath for a period of time. After the reaction was stable, the digestion was carried out in a microwave oven. For example, an onll salad oil plus an on, l nitric acid was pre-digested in a boiling water bath 20:1, in After that, add 2 to 5., a hydrogen peroxide solution, digestion in the microwave digestion furnace 5.llin that is completely digested. The same method of digesting sausages, peanut butter, sesame seeds, and fat was also satisfactory.
In general, the application of digester is very common, and the advantages in the process of substance digestion are also very common. At present, microwave digestion technology has been widely used in the analysis of geology, metallurgy, coal, petroleum, bad insurance and other fields. For the characteristics of biological samples, the commonly used microwave digestion method was reformed and satisfactory results were achieved.
October 30, 2024